The spectral calibration of the single-lens imaging spectrometer was studied. The relative relationship between the wavelength and the image plane was marked by the frequency sweep of the monochromator, and the characteristic spectral line of the mercury lamp was used for verification. According to the selected single lens model, the calibration scheme and steps were designed. An experimental platform was built according to the scheme, and the experimental analysis and verification were completed. The final results show that the calibration method in this paper is suitable for single-lens imaging spectral equipment.