The orientation of hydroxyapatite whiskers (HAw) is one of key to design and develop bioactive ceramics for bone regeneraction. Guided behavior based on the construction of magnetic anisotropy. In this study, magnetic nanoparticles Fe3O4 were loaded onto the surface of HAw by hydrothermal method, and magnetic HAw composites with excellent magnetic response characteristics were successfully prepared. The physicochemical properties and biological properties of magnetized HAw were systematically studied, and its orientation behavior in weak magnetic field was explored. The results show that ferric oxide (Fe3O4) nanoparticles are successfully loaded onto the surface of HAw, and the magnetized HAw has superparamagnetism and good magnetic response at room temperature, which can be manupulated by conventional permanent magnets. The alignment direction applied by the external magnetic field is effectively fixed by combining the magnetic field-induced grouting molding technology.The results of CCK-8 experiment show that magnetized HAw has no cytotoxicity.