Journal of Innovative Optical Health Sciences, Volume. 12, Issue 3, 1950014(2019)
Fringe optimization for structured illumination super-resolution microscope with digital micromirror device
Structured illumination microscopy (SIM) is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly used fluorescent labeling methods. Structured illumination can be obtained by either laser interference or projection of fringe patterns. Here, we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device (DMD) which can be directly attached to most commercial inverted fluorescent microscopes and update it into a SIM system. The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured light field were studied. With the optimized fringe pattern, 1:6× resolution improvement could be obtained with high-end oil objectives. Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated. Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in the field of life science and medicine.
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Xibin Yang, Qian Zhu, Zhenglong Sun, Gang Wen, Xin Jin, Linbo Wang, Jialin Liu, Daxi Xiong, Hui Li. Fringe optimization for structured illumination super-resolution microscope with digital micromirror device[J]. Journal of Innovative Optical Health Sciences, 2019, 12(3): 1950014
Received: Feb. 2, 2019
Accepted: Apr. 6, 2019
Published Online: Sep. 4, 2019
The Author Email: Xiong Daxi (xiongdx@sibet.ac.cn)