In order to analyze the accuracies of the color measurement for pillars pattern of holographic paper with different spectrophotometers, the X-Rite 7000A integrating sphere, X-Rite MA68Ⅱ multi-angle and X-Rite Spectro Eye45/0 three different instruments with different geometries are selected to measure the positions of along and perpendicular to the pillars. The data analysis indicates that the X-Rite 7000A with the large area view and specular component includes mode shows the smaller spectral mean square error on different positions. The chromaticity values of holographic paper along the pillars measured by X-Rite 7000A are similar while perpendicular to the pillars the chromaticity values fit a good linear relationship. It is found that the chromaticity values are very similar with the same position by rotating 180° and the graving direction of the grating can be deduced by the chromaticity values from different positions perpendicular to the pillars.