CsPbI3 quantum dots doped borosilicate glasses were prepared by a high-temperature melting-heat treatment technology with Cs2O, PbI2 and NaI as precursors. According to the results by X-ray diffraction and transmission electron microscopy, CsPbI3 quantum dots are precipitated in the glass substrate. The emission peak and absorption edge appear a red shift, the photoluminescence intensity and quantum yield firstly increase and then decrease, and the photoluminescence decay lifetime of quantum dots gradually increases with increasing the heat-treatment temperature or B2O3 concentration. Also, the two-dimensional (2D) network structure in the glass increases, and the mobility of Cs+, Pb2+ and I- ions increases with the increase of B2O3 concentration, which is beneficial for the precipitation of CsPbI3 quantum dots and the passivation of surface defect. The tunable red light emission of CsPbI3 quantum dots doped borosilicate glass could have potential applications in the visible band lasers and white light-emitting diodes (LED).