Factor group symmetry analysis method and position symmetry analysis method were applied to analyze the lattice vibration of Ca3 (BO3 )2 crystal. The fundamental lattice vibrational modes of Ca3 (BO3 )2 can be subdivided into external modes (3A1g+4A2g+7Eg+3A1u+3A2u+6Eu) and internal modes (2A1g+2A2g+4Eg+2A1u+2A2u+4Eu). Total vibrational modes are classified as 5A1g+6A2g+11Eg+5A1u+6A2u+11Eu. Among them, the ungerade modes A2u+Eu are acoustic modes, 5A2u+10Eu are IR active, the gerade modes 5A1g+11Eg are Raman active, and the others are neither Raman active nor IR active. Ca3 (BO3 )2 powder was synthesized by solid state method. Room temperature Raman spectrum of the powder was obtained. The experiment results are discussed, and the characteristic vibrational peaks of the BO3-3 groups were identified.