Journal of Innovative Optical Health Sciences, Volume. 17, Issue 2, 2450001(2024)

Geometric regulation of collective cell tangential ordering migration

Hao Dong1, Yuming Zhou1, Xuehe Ma1, Junfang Liu1, Fulin Xing1, Jianyu Yang1, Qiushuo Sun1, Qingsong Hu1, Fen Hu1、*§, Leiting Pan1,2,3,4、**§, and Jingjun Xu1,3
Author Affiliations
  • 1The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. China
  • 2State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, P. R. China
  • 3Shenzhen Research Institute of Nankai University, Shenzhen, Guangdong, 518083, P. R. China
  • 4Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi 030006, P. R. China
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    Collective cell migration is a coordinated movement of multi-cell systems essential for various processes throughout life. The collective motions often occur under spatial restrictions, hallmarked by the collective rotation of epithelial cells confined in circular substrates. Here, we aim to explore how geometric shapes of confinement regulate this collective cell movement. We develop quantitative methods for cell velocity orientation analysis, and find that boundary cells exhibit stronger tangential ordering migration than inner cells in circular pattern. Furthermore, decreased tangential ordering movement capability of collective cells in triangular and square patterns are observed, due to the disturbance of cell motion at unsmooth corners of these patterns. On the other hand, the collective cell rotation is slightly affected by a convex defect of the circular pattern, while almost hindered with a concave defect, also resulting from different smoothness features of their boundaries. Numerical simulations employing cell Potts model well reproduce and extend experimental observations. Together, our results highlight the importance of boundary smoothness in the regulation of collective cell tangential ordering migration.

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    Hao Dong, Yuming Zhou, Xuehe Ma, Junfang Liu, Fulin Xing, Jianyu Yang, Qiushuo Sun, Qingsong Hu, Fen Hu, Leiting Pan, Jingjun Xu. Geometric regulation of collective cell tangential ordering migration[J]. Journal of Innovative Optical Health Sciences, 2024, 17(2): 2450001

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    Paper Information

    Category: Research Articles

    Received: Jan. 2, 2024

    Accepted: Jan. 21, 2024

    Published Online: Apr. 19, 2024

    The Author Email: Hu Fen (hufen@nankai.edu.cn), Pan Leiting (plt@nankai.edu.cn)

    DOI:10.1142/S1793545824500019

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