Journal of Innovative Optical Health Sciences, Volume. 16, Issue 3, 2244007(2023)

SOFFLFM: Super-resolution optical fluctuation Fourier light-field microscopy

Haixin Huang1,*... Haoyuan Qiu1, Hanzhe Wu1, Yihong Ji1, Heng Li1,2, Bin Yu1, Danni Chen1 and Junle Qu1 |Show fewer author(s)
Author Affiliations
  • 1College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, P. R. China
  • 2Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen 518055, P. R. China
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    Fourier light-field microscopy (FLFM) uses a microlens array (MLA) to segment the Fourier plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional (3D) structure of the sample using 3D deconvolution calculation without scanning. However, the resolution of FLFM is still limited by diffraction, and furthermore, it is dependent on the aperture division. In order to improve its resolution, a super-resolution optical fluctuation Fourier light-field microscopy (SOFFLFM) was proposed here, in which the super-resolution optical fluctuation imaging (SOFI) with the ability of super-resolution was introduced into FLFM. SOFFLFM uses higher-order cumulants statistical analysis on an image sequence collected by FLFM, and then carries out 3D deconvolution calculation to reconstruct the 3D structure of the sample. The theoretical basis of SOFFLFM on improving resolution was explained and then verified with the simulations. Simulation results demonstrated that SOFFLFM improved the lateral and axial resolution by more than 2 and 2 times in the second- and fourth-order accumulations, compared with that of FLFM.

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    Haixin Huang, Haoyuan Qiu, Hanzhe Wu, Yihong Ji, Heng Li, Bin Yu, Danni Chen, Junle Qu. SOFFLFM: Super-resolution optical fluctuation Fourier light-field microscopy[J]. Journal of Innovative Optical Health Sciences, 2023, 16(3): 2244007

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    Paper Information

    Category: Research Articles

    Received: Jul. 22, 2022

    Accepted: Nov. 9, 2022

    Published Online: May. 25, 2023

    The Author Email:

    DOI:10.1142/S1793545822440072

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