A kind of terahertz metamaterial with electromagnetically induced transparency (EIT) like resonance and its application in streptavidin-agarose (SA) specific sensing are demonstrated. Finite element method is used to analyze the high Q EIT-like resonance and its sensing performance. The label-free specific biosensor is composed of metamaterials functionalized by biotins and octadecanthiols for SA biorecognition experiments. The transmission properties are measured by a high-resolution backward-wave oscillator (BWO) spectral system. The results show that the EIT-like resonance of the metamaterial sensor has a high Q factor of 34 and a sensitivity of 24.7 GHz/RIU. Sensitivity of the resonance frequency shift to SA concentration is 0.65 GHz/(mg/mL). The results provide references for applying terahertz devices to label-free biological and chemical sensing.