Terahertz(THz) wave has great development potential in biomedical science due to its nonionizing, labelless and real-time characteristics. To distinguish biological tissues with similar permittivity, a metamaterial with Fano resonance is designed and fabricated for THz reflection imaging.The simulation and experimental results show that the reflection sensitivity of the metamaterial is132 GHz/RIU at 0.82 THz. The metamaterial not only enhances the contrast of imaging resolution plate,but also improves the contrast between the muscle and the fat tissues by approximately 7.5%, therefore,it can improve the contrast of biological tissue slice images. This work has established an experimental and theoretical foundation for future terahertz applications in clinical tissue samples.