Application experiments of holographical-speckle method for intervertebral disc measurement of offset variable and metamorphosis in orientation of the lumbar surface are performed. The instruction of specimen making, loading device, design of beam path and process of experiment are introduced. The results of experiment indicate that holographical-speckle method can obtain three dimensional (3D) offset variable field of intervertebral disc, and metamorphosis can be got by calculation. This method has high accuracy, full-field and noncontact. The results of experiment offer data for contrasting the stabilization of different spinal fixation method.