To address the shortcomings of the existing oscilloscope in laboratory, such as poor integration and weak scalability, and to improve the efficiency and accuracy of mass spectrum signal acquisition, a LabVIEW-based signal acquisition and processing system for mass spectrometry and infrared spectrum is designed. Based on the LabVIEW software platform and CSE22G8 data acquisition card, the designed system can realize real-time acquisition and processing of weak mass spectrometry signals under the background with low signal-to-noise ratio using the superimposed average denoising algorithm. In addition, the synchronous control of tunable optical parametric oscillator (OPO) is realized through TCP communication protocol, so that the user can obtain the corresponding infrared absorption spectrum while collecting mass spectrum signals. Tests show that the resolution of the system is doubled compared with the original oscilloscope in laboratory, the number of sampling points can reach 2000 per microsecond, and the waveform reconstruction ability is significantly improved, indicating that the system has better data acquisition performance.