Determination of the geographic origin of blue Sapphire is important in gemological research. Metamorphic Sapphires from Sri Lanka and basalt-related Sapphires from Laos are tested. UV-Vis-NIR chemical analysis fits the basic characteristics of Metamorphic and basalt-related Sapphires. Three-dimensional fluorescence spectroscopy revealed distinct fluorescence at around 420 to 440 nm, 540 to 560 nm, 560 to 580 nm and 694 nm of Metamorphic Samples. Meanwhile, basalt-related samples present fluorescence at 418 nm. In this research, the main difference between the two types of Sapphires is that metamorphic samples present strong fluorescence. Besides, fluorescence at 540 to 560 nm, 560 to 580 nm occurs only in metamorphic Sapphires.