Li+, Bi3+ doped Lu2O3∶Ho3+, Yb3+ powders were prepared by high temperature solid state method. The microstructure of the synthesized powder was analyzed by X-ray diffractometer. The morphology and size of the sample were observed by field emission scanning electron microscopy. The up conversion emission spectra and energy level lifetime of the synthesized powders were analyzed by UV-Vis near-infrared fluorescence spectrometer. The results show that Li+, Bi3+ doped Lu2O3∶Ho3+, Yb3+ powders still maintain the Lu2O3 cubic phase structure. After Li+ or Bi3+ doped, the dispersion of the synthesized powders is better, the particles are more uniform and closer to the sphere, and the particle size of Li+ doped powders increases obviously. Under the excitation of 980 nm, the green light intensity of Ho3+ in the 4%Li+ or 1.5%Bi3+doped powders is increased by about 3.9 times and 2.8 times, respectively. With the increase of Li+ concentration, the 5S2 energy level lifetime of Ho3+ in the synthetic powders increase first and then decrease, while with the increase of Bi3+ concentration, the energy level lifetime of synthetic powders are gradually shortened.