Acta Optica Sinica, Volume. 37, Issue 3, 318013(2017)
Airy Beam Light Sheet Microscopy with Deconvolution
In order to form an extended light sheet to provide high resolution across a large field of view (FOV), the combination of Airy beam light sheet illumination and deconvolution arithmetic is proposed. The light intensity distributions of Gaussian beam and Airy beam in the focal plane of the objective are numerically simulated. The light sheet microscope setup is constructed. Airy beam is created by modulating the wavefront with a cubic polynomial function, and fluorescent microspheres and stained zebrafish musculature are imaged. Finally, based on the pictures imaged by Airy light sheet microscopy, deconvolution arithmetic is built to improve the axial resolution. For fluorescent microspheres (the amplification is 42), the FOV is extended from 25 μm to 208 μm. For labeled zebrafish musculature (the amplification is 53), the FOV is extended from 20 μm to 167 μm.The experimental result and numerical simulation all suggest that the FOV of the system can be extended with high resolution.
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Xu Hao, Zhang Yunhai, Zhang Xin, Xiao Yun, Huang Wei. Airy Beam Light Sheet Microscopy with Deconvolution[J]. Acta Optica Sinica, 2017, 37(3): 318013
Received: Sep. 12, 2016
Accepted: --
Published Online: Mar. 8, 2017
The Author Email: Hao Xu (18552441906@163.com)