In order to screen and identify the source of spilled oils at sea, synchronous fluorescence scans combined with clustering analysis are proposed and applied to different crude oil and weathering crude oil. SFS data of Δl=25 nm were recorded and dealt with clustering analysis. The cluster results of SFS data in the range of 300～500 nm show that the crude oil and the weathering oil could separate completely. And the crude oils from different sea areas, also collected at different time, clustered into different groups, respectively. The results indicate that this method could preliminarily selected, and maybe serves as an assistant method in oil spill identification.