Acta Optica Sinica, Volume. 41, Issue 21, 2111001(2021)
Study of Multi-Resolution Microscopic Correlation Imaging Based on Optimized Hadamard Matrix
A multi-resolution microscopic correlation imaging method based on a digital micromirror device was proposed to meet the diverse needs of microscopic imaging and resolve the contradiction between imaging quality and imaging time in practical applications. In this method, an LED was used as the background illumination source, and the original optical path of a research-grade upright fluorescence microscope was modified to be an optical path of correlation imaging. The multi-resolution optimized Hadamard matrix was adopted as the preset pattern of the digital micromirror device. Continuous multi-resolution microscopic imaging of biological tissue samples was achieved by this system. The experimental results demonstrate that the resolution of the multi-resolution microscopic correlation imaging system reaches 218 nm. Eight groups of images in different resolutions can be output simultaneously after a single measurement. Therefore, different resolutions can be selected according to particular image quality requirements in practical applications. By reducing the imaging time and storage space needed, this system greatly improves the flexibility of microscopic imaging. The proposed multi-resolution microscopic correlation imaging method can be extended to fields such as cell screening and real-time cell imaging. It is expected to promote the application of correlation imaging in microscopic imaging of cells and biological tissues.
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Yueshu Feng, Cheng Zhou, Xuan Liu, Xiaohan Liu, Lijun Song. Study of Multi-Resolution Microscopic Correlation Imaging Based on Optimized Hadamard Matrix[J]. Acta Optica Sinica, 2021, 41(21): 2111001
Category: Imaging Systems
Received: Apr. 6, 2021
Accepted: May. 17, 2021
Published Online: Oct. 29, 2021
The Author Email: Song Lijun (ccdxslj@126.com)