Acta Optica Sinica, Volume. 41, Issue 7, 0706004(2021)
Immunodetection of Alpha-Fetoprotein Based on Dual-Channel Probe Type 81° Tilted Fiber Grating
In this study, a dual-channel probe type 81° tilted fiber grating (81°TFG) sensor is proposed and used for the evaluation of alpha-fetoprotein (AFP) immunoassays. First, the end face of 81°TFG is plated with a silver film to form the reflective type 81°TFG, and a dual-channel sensor is constructed by a 2×2 coupler and two 81°TFG probes. Then, large-size AuNs and GO are modified on the grating surface, and AFP monoclonal antibodies are used as the specific recognition unit to modify the grating surface to achieve a dual-channel probe type 81°TFG local surface plasmon resonance (LSPR) AFP immunosensor. Finally, the immune detection experiment is completed in a complex serum environment. The results demonstrated that the detection limit of the sensor for the mass concentration of AFP antigen is within the range of 1--10 pg/mL, the detection saturation point and sensitivity are ~200 ng/mL and ~0.155 nm/log(pg/mL), respectively, it has a satisfactory specific response to the serum of liver cancer patients is observed. Compared with the single-channel transmission type 81°TFG sensor, the sensor could simultaneously control the detection of positive and negative samples of the target molecules; therefore, enhancing the reliability in clinical detection applications, and the operation is simpler and the application potential is greater.
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Qingming Lü, Binbin Luo, Qi Yang, Yang Zhang, Shengxi Wu, Zhipeng Gu, Shenghui Shi, Decao Wu, Taojiang Wu, Mingfu Zhao. Immunodetection of Alpha-Fetoprotein Based on Dual-Channel Probe Type 81° Tilted Fiber Grating[J]. Acta Optica Sinica, 2021, 41(7): 0706004
Category: Fiber Optics and Optical Communications
Received: Sep. 27, 2020
Accepted: Nov. 23, 2020
Published Online: Apr. 11, 2021
The Author Email: Luo Binbin (luobinbin@cqut.edu.cn)