Journal of Atmospheric and Environmental Optics, Volume. 7, Issue 1, 71(2012)
Phase-Locking Detection Technique of Fluorescence Lifetime
As the intrinsic parameter of fluorescence signal, the accurate measurement of fluorescence lifetime is very important to fluorescence detecting and analyzing technique. According to the characteristics of fluorescence signal and definition of fluorescence lifetime, principle of phase-locking detection technique of fluorescence lifetime is introduced. Based on phase locked loop circuit, fluorescence lifetime detecting system is designed, which is used to dissolved oxygen detecting with fluorescence method. The experimental results showed that the relative error of measured values and actual values is less than 1.5%, and the response time is less than 60 s.
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GUO Li-quan, ZHANG Yu-jun, YIN Gao-fang, ZHANG Kai, WANG Zhi-gang. Phase-Locking Detection Technique of Fluorescence Lifetime[J]. Journal of Atmospheric and Environmental Optics, 2012, 7(1): 71
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Received: May. 4, 2010
Accepted: --
Published Online: Jan. 16, 2012
The Author Email: Li-quan GUO (lqguo@aiofm.ac.cn)