In order to quantify the spectral data of a miniature infrared spectrometer based on hollow planar waveguide, we built a wavelength calibration device based on carbon dioxide (CO2) laser and integrating sphere on the basis of analyzing the common wavelength calibration method used in the laboratory. The spectrum calibration range of the device is wide and the resolution is high, which overcomes the shortage of traditional laboratory calibration methods and improves the calibration precision. We first introduce the working principle of the infrared spectrometer based on hollow planar waveguide, then use the calibration device to calibrate the wavelength of the spectrometer, and then complete the calibration data analysis by the polynomial fitting algorithm. Finally, the calibration results are verified by the measurement of two narrow band filters. The experimental results show that the calibration error of the central wavelength is not more than 0.02 μm and the spectral resolution can reach 144 nm by using the combination of CO2 laser and integrating sphere.