ICP-AES technique was used to determine the mineral elements in Bupleurum at different habitat. The results show that: (1) In Bupleurum, the content and accumulation of K was the highest among 5 macroelements, the content and accumulation of Fe was the highest among 5 microelements. (2) In Bupleurum, the content of Ca, Mg, P, Na and Cu was high in habitat of Beijing, the content can respectively reach to 6.40, 3.84, 3.45, 4.97 mg·g-1, and 25.20 μg·g-1; while the content of K, Ca, Mg, P, Zn, Mn and Cu was low in habitat of Wanrong, and the content was only 12.43, 4.57, 1.92, 1.79 mg·g-1 and 50.04, 32.21, 15.43 μg·g-1 respectively. (3) In Bupleurum, the content of P∶K, Zn∶Fe, Cu and Mn was significantly different at different habitat, while Mg and Ca showed little difference. Conclusion: In Bupleurum, the content, accumulation and proportion of mineral elements were differenct at different habitat.