A new methamidophos-specific molecularly imprinted polymer (MIP) was synthesized based on non-covalent interaction.The complexes formed between MAP and MAA were evaluated by 1H NMR,FTIR and UV spectrometry.The MAP-MAA complexes of 1:2 mole ratio were obtained by cooperative hydrogen bonding and the complexes possessed better stabilization (K＝2.894×106 L²·mol^-2 ).The Infrared spectrometry differences of the MIPs before and after saturated with MAP further indicated that there were carboxyl functional groups in the MIP,which could interact with the template by cooperative hydrogen bonding specifically.