Due to the temperature sensitivity of semiconductor materials, the photovoltaic response output characteristics of photovoltaic cells will change with the temperature variations caused by laser irradiation. This paper focuses on the interference of repetition frequency laser irradiation on the response characteristics of silicon-based photovoltaic cells. Starting from the principle of photovoltatic effect, based on the response output model and one-dimensional heat conduction equation, the effects of different pulse parameters on the voltage output of photovoltaic cells under laser irradiation are calculated. The results indicate that repetition frequency lasers can significantly impact the normal operation of photovoltaic cells. However, by controlling the pulse energy and selecting appropriate parameters, such as pulse width and pulse interval, significant interference effects can be achieved.