To obtain the ultraweak luminescence properties of plant affected by multilateral growth, environment factors of osmanthus tree seedings were used as the research material.Quadratic rotation-orthogonal composite experimental design was used to study the effects of illumination,temperature,relative humidity,and moisture on the ultra-weak luminescence of leaves of osmanthus tree seedings.The model of four environmental factors to ultra-weak luminescence of osmanthus tree seedings leaves was established.The ANOVA(analysis of variance) analysis showed that the significant value of influence of different factors and their interaction on ultra-weak luminescence are different.The linear effects of four variables,the quadratic effects of illumination,relative humidity and moisture,and the interaction effects of illumination & moisture and temperature & relative humidity are all significant,with p-value ≤0.01.And when light by 10 stick of lamp tube (intensity of illumination value 4 229 Lx) and mean temperature,humidity and water respectively controlling in 35 ℃ 90 mL/d,85%, the value of ultraweak luminescence was maximum.Based on that,the influence rule of each influential environmental factors and their interactions on the ultra-weak luminescence was also included.