Research is done on the single-photon interference with two different Mach-Zehnder (M-Z) interferometers-particle and fiber separately. Achieving the high contrast above 90%, the experiment approves the routing control of single photon with Mach-Zehnder interferometers of particle and fiber successfully. Pulse-modulating and attenuating techniques are implemented to get the single photon, and the subsingle-photon source is achieved with only 0.1 photon in one pulse. The routing control of the single photon is under the control of the changing of PZT power. Furthermore, the synchronous single-photon coincidence detection technique is used to detect the single-photon, modulation with repeating frequency of 10 kHz and pulse FWHM of 100 ns, the high sensitive detection is improved with high quantum efficiency over 70% and the dark count below 0.2 s-1, single-photon output through the chosen path with given chosen PZT power has been observed. The experiment verifies that single-photon routing control can be achieved in Mach-Zehnder interference.