Spectroscopy and Spectral Analysis, Volume. 35, Issue 8, 2217(2015)
Determination of H2S in Rat Intestinal Perfusion Solution Based on Fluorescence Analysis
Under alkaline conditions,Fluorescein mercury has strong fluorescence,however,when it met S2-,its fluorescence would quench,in view of the above,a fluorescence method for determination of H2S in biological samples was established.In the 0.1 mol·L-1 NaOH dilution,when the concentration of fluorescein Mercury and Na2S was 5.0×10-5 and 1.0×10-5 mol·L-1 respectively,the fluorescence intensity of system was determined at 522 nm.The results showed that,at the range of 4.0×10-7~2.0×10-6 mol·L-1,the concentration decreasing of H2S and fluorescence intensity had good linear relationship,r=0.998 0,the RSD of precision test was 4.59% (n=7),the detection limit was 3.5×10-8 mol·L-1,the content of H2S in the sample were 1.01×10-6 and 1.15×10-6 mol·L-1,and the recovery rate was 95.8%~101.0%,the method has the advantages of simple operation,high sensitivity,good selectivity,can accurately determine of H2S in intestinal perfused solution,and provides the basis for the determination of endogenous H2S.
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HOU Jun-feng, LI Xin-xia, SHEN Xue-ru, Miliban Huojia, GUAN Ming. Determination of H2S in Rat Intestinal Perfusion Solution Based on Fluorescence Analysis[J]. Spectroscopy and Spectral Analysis, 2015, 35(8): 2217
Received: Jun. 8, 2014
Accepted: --
Published Online: Aug. 25, 2015
The Author Email: Jun-feng HOU (1097789976@qq.com)