The ABS resin wastewater was treated by biological activated carbon and sequencing batch reactor(BAC-SBR), and the variation trend of three-dimensional fluorescence spectra, chemical oxygen demand (COD) and total organic carbon of (TOC) for ABS resin wastewater was detected in the treatment process. The difference of fluorescence fingerprint for ABS resin wastewater and typical municipal wastewater was studied comparatively. The correlation of total fluorescence intensity removal ratio with the removal ratio of COD and TOC for ABS wastewater was studied intensively in the treatment process. The results show that the fluorescence fingerprint (the ratio of Peak A/Peak B) of ABS wastewater is 0.124, which is much lower than that of typical municipal wastewater (about 1.6). In the whole treatmet process, the total fluorescence intensity removal ratio has good linear relation with the removal ratio of COD and TOC, and their corelation coefficient were 0.98 and 0.99, respectively. Therefore the removal ratio of the aromatic organic pollutants in wastwater could be analysed by the determination of the change of fluorescence intensity.