To obtain materials with superior antibacterial properties, Ag-ZnO/Talc ternary composite was prepared by a water-bath heating method with black talc (from Guangfeng, Jiangxi) as a matrix material. The composite was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy, respectively. Escherichia coli and staphylococcus aureus were used as the experimental strains. The antibacterial properties of the composite were examined by a plate counting method and a bacteriostatic zone method. The results show that the layered structure of talc sheet promotes the dispersion of nanoparticles and increases the contact area between the composite and bacteria. The Ag-ZnO/Talc composite displays a high antibacterial activity, and the antibacterial rate against escherichia coli and staphylococcus aureus reaches more than 99%.