Acta Optica Sinica, Volume. 40, Issue 18, 1806001(2020)
An Immunosensor Based on the Graphene-Oxide-Encapsulated Au-Nanoshell-Coated Long-Period Fiber Grating
In this study, a novel immunosensor based on graphene-oxide (GO)-encapsulated Au-nanoshell (EGO-AuNS)-coated long-period fiber grating (LPFG) is presented for avian influenza virus (AIV) detection. The EGO-AuNS composites were obtained by coating GO on the surface of AuNS via electrostatic bonding, and immobilized on the surface of LPFG via covalent bonding using a silane coupling agent. Then, AIV monoclonal antibodies (AIV-MAbs), which serve as specific biomolecule recognition units, were fixed on the grating surface to realize the EGO-AuNS-LPFG immunosensor. The experimental results indicate that the refractive index (RI) sensitivity of the EGO-AuNS-LPFG immunosensor is -66.60 nm/RIU when RI is from 1.333 to 1.411, which is six times greater than that of the LPFG-based sensor. Based on the detection of AIV antigen solutions with different concentrations, the limit of detection of the immunosensor is observed to be approximately 8 ng/mL. Further, the immunosensor becomes saturated at approximately 50 μg/mL and exhibits a linearity sensitivity of approximately 2946.25 pm/(μg·mL -1), which is 7.3 times greater than that of the GO-coated cladding-etched LPFG immunosensor. Based on the detection results of several allantoic fluids, the proposed immunosensor exhibits good specificity and clinical effectiveness. Furthermore, it exhibits considerable promise for application in the biomedical field.
Get Citation
Copy Citation Text
Shenghui Shi, Decao Wu, Xin Wang, Qinglin Nie, Zhijiang Liu, Binbin Luo, Enhua Liu, Peng Liu, Mingfu Zhao. An Immunosensor Based on the Graphene-Oxide-Encapsulated Au-Nanoshell-Coated Long-Period Fiber Grating[J]. Acta Optica Sinica, 2020, 40(18): 1806001
Category: Fiber Optics and Optical Communications
Received: Apr. 20, 2020
Accepted: Jun. 9, 2020
Published Online: Aug. 27, 2020
The Author Email: Luo Binbin (luobinbin@cqut.edu.cn), Zhao Mingfu (luobinbin@cqut.edu.cn)