PbSe quantum dots (QDs) in the sodium- aluminum- borosilicate silicate glass are successfully prepared by using a melting method of two- step heat treatment. The size of obtained QDs distributes within 3.63~4.33 nm and with the doped volume ratio of 2% in the glass. The optimal heat treatment temperature is determined by using a differential thermal analysis. The crystallization, particle size and distribution of the QDs in the glass are observed by X- ray diffractometer and transmission electron microscopy. The absorption and photoluminescence (PL) spectra of the QDs are also measured by ultraviolet (UV)-visible-near infrared (NIR) spectrophotometer and fluorescence spectrometer, respectively. There is evidence to show that only when the first heat-treatment time is between 3~5 h and the temperature is 500 °C, the glass has PL emission, ranging in 1220~1279 nm and with full width at half maximum (FWHM) of 200 nm. For the second heat treatment, the optimal time and temperature are 10 h and 540 °C, respectively. An empirical formula is given for the PbSe QD size depending on the first heat treatment time. The method of PbSe QD doped glass can help to form QD doped fiber devices with high gain in the future.