A method for determining 30 trace elements in human plasma was developed by using microwave digestion-ICP-MS. Under the optimized conditions the samples were analyzed by ICP-MS after microwave digestion. The double internal standard elements Rh and Re were used to compensate for matrix suppression effect and sensitivity drift. The analytical results showed that the detection limits of the 30 trace elements were in the range of 0.01-0.68 ng·mL-1. The recovery rates of the studied elements were from 85% to 119%. The relative standard deviations (RSD) of the method were in the range of 1.7%-10.2%. The national standard reference material GBW 09131 was analyzed by the described method and the measured element values accorded with the standard values. It is indicated that the method is rapid, simple and accurate for determining trace elements in human plasma.