A single opto-electrical and radar integration system was designed and the feasibility to apply the system to measurement of three dimensional attitudes of flying targets was discussed. Then,the principles,algorithms and measuring errors of the system were researched. According to three-dimensional attitude computer methods, the system's key technical indicators were determined, and the effective distance and the attitude measurement accuracy of the system for a simulated target was analyzed. The results show that the effective distance to the simulated target distance is more than 60 km,and the real-time three-dimensional attitude measurement accuracy is less than 120′. By use of the exiting device,a single-station measurement experiment to the real-time three-dimensional attitude of the target was performed and the results show it is feasible to measure the three-dimensional attitudes of targets by single opto-electrical and radar integration instruments , which not only can improve measuring accuracy and working efficiency and also can eliminate the restriction for measuring instrument distribution.