In the manufacturing process of high-resolution mobile phone lens consisted of glass and plastic material, many problems of assembly between glass lens and plastic lens group were existed such as sensitive to position change, low efficiency and low yield. The optimal proposal based on multi-point infinite conjugation modulation transfer function measurement was adopted to lens alignment and assembly in real-time feedback. As a critical imaging component of chart acquisition for modulation transfer function calculation, performance of multi-axis focusing lens played a significant role in guaranteeing measurement accuracy. The relationship between the design parameters of multi-axis focusing lens and the optical parameters of mobile phone lens to be assembled was established. Multi-configuration setting and aberration optimization of multi-axis focusing optical system were realized by ZEMAX simulation software. Simulation results showed that the size of focusing lens was compact, and both its tangential and sagittal modulation transfer function curves were nearly coincided with diffraction limit. The designed multi-axis focusing lens theoretically could be flexibly exploited to multi-point modulation transfer function measurement and precise assembly of high-resolution mobile phone lens up to 48 megapixel, which maximum full field of view angle reached 106°.