Through manganese doping, the luminescence wavelength of perovskite can be adjusted to improve its quantum yield. In this paper, Mn²⁺ doping was lead into two-dimensional perovskite (PMA)₂PbBr₄ and the luminescence properties and energy transfer mechanism in Mn-doped two-dimensional perovskite (PMA)₂PbBr₄ were studied. The results show that after manganese doping, the exciton recombination luminescence peak (419 nm) of the original two-dimensional perovskite changed into a transition (608 nm) between the Mn²⁺ orbital ⁴T₁→⁶A₁, in which an effective energy transfer occurred. The introduction of Mn²⁺ leads to a certain degree of lattice distortion, and then the exciton lifetime decreases (from 6.51 ns to 0.30 ns); at the same time, the luminescence lifetime brought by Mn²⁺ is not sensitive to the doping concentration (0.23 ms), because the luminescence corresponds to the transition between ⁴T₁ and ⁶A₁ of Mn²⁺ at this time. After manganese doping, its optical stability has also been significantly improved. Therefore, manganese doping is an effective means to adjust the wavelength or bandwidth of two-dimensional perovskite and improve the luminous efficiency, which provides the possibility for the application of two-dimensional perovskite in white light lighting.