Journal of Innovative Optical Health Sciences, Volume. 17, Issue 2, 2350022(2024)
Three-dimensional morphological and fluorescent imaging of zebrafish with a continuous-rotational light-sheet microscope
Light-sheet fluorescence microscopy (LSFM) has been widely used to image the three-dimensional (3D) structures and functions of various millimeter-size bio-specimen such as zebrafish. However, the sample adsorption and scattering cause shading of the light-sheet illumination, preventing the even 3D image of thick samples. Herein, we report a continuous-rotational light-sheet microscope (CR-LSM) that enables simultaneous 3D bright-field and fluorescence imaging. With a high-accuracy rotational stage, CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles. Then, 3D morphology and fluorescent structure were reconstructed with a developed algorithm. Using CR-LSM, zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.
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Dongmin Zhang, Guang Yang, Yao Tan, Chong Chen, Jie Zhang, Hui Li. Three-dimensional morphological and fluorescent imaging of zebrafish with a continuous-rotational light-sheet microscope[J]. Journal of Innovative Optical Health Sciences, 2024, 17(2): 2350022
Category: Research Articles
Received: May. 11, 2023
Accepted: Jul. 12, 2023
Published Online: Apr. 19, 2024
The Author Email: Li Hui (hui.li@sibet.ac.cn)