In recent years, the issue of excessive antibiotic residues in the poultry egg industry has drawn considerable attention as a serious food safety concern. Currently, immunoassay, high-performance liquid chromatography, and microbial detection are the three primary techniques used to detect antibiotic residues in livestock and poultry products. When it comes to quick on-site sample testing, these technologies do have certain drawbacks. This study established a quick, accurate, and fast approach for detecting antibiotic residues in eggs using laser-induced fluorescence analysis. The antibiotics that might be found in chicken eggs were chosen for the samples, including ciprofloxacin, norfloxacin, ofloxacin, tetracycline, and gentamicin. The samples were created using egg white as the solvent. Antibiotic reagents were applied to imitate antibiotic residues in eggs, and the fluorescence spectra of antibiotics at various doses were examined. Euclidean distance and probability density approaches were used for the qualitative analysis, which had a 100% accuracy rate within three standard deviations. After examining samples at different concentrations, each antibiotics calibration curves (c-S curves) were created. The results showed that within the effective detection range (ciprofloxacin: 0. 000 1 to 1 mg·mL-1, norfloxacin: 0. 000 2 to 1 mg·mL-1, ofloxacin: 0. 000 05 to 1 mg·mL-1, tetracycline: 0. 000 1 to 0. 2 mg·mL-1, chloramphenicol: 0. 01 to 1 mg·mL-1), the antibiotic content could be calculated more accurately based on the net peak area of the fluorescence spectra. Ultimately, the precision and detection bound of the antibiotic quantitative curve were computed, suggesting that the technique is applicable to detect antibiotic residues in eggs; however, the high detection limit remains an issue, necessitating additional investigation to lower the detection limit.