The atmospheric ultraviolet imaging spectrograph (AUVIS) is an ultraviolet imaging spectrograph that uses a two-dimensional charge-coupled device detector to collect both the spectrum and the swath perpendicular to the flight direction. The design of the system components, the principle, and the main specifications of the AUVIS are introduced. The calibration theory was studied. Considering the characteristics of the AUVIS, a PtNe hollow cathode lamp was chosen as the calibration source, a calibration setup was established, and the AUVIS was calibrated. After the experimental data processing, a function of pixel number-wavelength was established. The uncertainty of the spectral calibration is 0.043 nm.