Chalcogenide glass of GeS2-Ga2S3-CdS system was prepared by heat inducting. The results of X-ray diffraction (XRD), transmission spectrum and scanning electron microscope (SEM) showed that transparent surface microcrystal glass containing CdGa2S4 has been gained. The second harmonic generation (SHG) effect of the microcrystal glass was studied by Maker-fringe technique, and the result indicated that CdGa2S4 in the glass induced the occurrence of SHG effect. The isotropy of the glass was broken when CdGa2S4 was growing preferentially on the surface of the microcrystal glass, and two enveloping Maker-fringes were obtained. And the maximum of relative second harmonic (SH) intensities appeared at incidence angle around ±(35°～50°) which was 8 times as that of α-quartz single crystal. When CdGa2S4 was growing non-preferentially on the surface of the microcrystal glass, only one enveloping Maker-fringe was obtained because of the strong scattering from CdGa2S4 grains. That is the maximum relative SHG intensities which appeared at incidence angle of 0.