The experimental center laboratory of biology carries a large number of experimental teaching activities. It has some characteristics, including large number of participants, large mobility, weak safety awareness, open sharing, and various types of courses, which is also easy to cause laboratory safety accidents. In order to ensure the safe and efficient operation of the experimental center laboratory of biology in the the whole year, an implementation path for the closed-loop management of the experimental center laboratory safety of biology is proposed, including hazard management, laboratory safety check, laboratory staffs, laboratory safety education, laboratory bio-safety, hazardous chemicals safety, laboratory waste safety and measures for handling laboratory safety accidents. The path provides guidance for the safe management of the experimental center of biology.