Real-time data acquisition is a basic and important link on industry and aviation measure and control realm.How to increase the real-time quality of data collection and saving is a problem concerned by technical personnel.A high speed data acquisition system which has been used recording the secondary planet interferespectrometer imaging based on 32 bit high speed data collection card ADLINK PCI 7300A is described. The realization of high-speed data collection, saving and real-time displaying based on vc6.0 and Windows NT is elaborated.In the process the multithreading,timer,doublebuffer and synchronous object etc have been used, the system stability and real-time quality are effectively assured, the function of high speed digital image card is realized by digital I/O card.