A 250 pJ seed pulse from 1030 nm fiber mode-locked laser is amplified by cryogenic Yb:YAG regenerative amplifier. After the seed pulse is amplified by 20 circles by controlling the electro-optical switch, the max single pulse energy is 217 μJ, the output rate is 10 Hz. Meanwhile, the spectrum decreases from 8.9 nm to 0.3 nm for gain narrowing, the corresponding pulse width is compressed from 18.0 ps to 5.5 ps, which fits the simulated result of 0.4 nm and 4.2 ps. The experimental result demonstrates that regenerative amplifier using gain narrowing can realize the require for pump pulse width and spectrum width of subpicosencond optical parametric chirped pulse amplification (OPCPA), and avoid high order nonlinear effect by fiber grating.