Electro static discharge (ESD) is a problem often met in liquid crystal display (LCD) production, which may produce in every process. Based on the actual situation met in the process, the reasons for ESD are studied. Through theoretical analysis and experimental verification, if there are many sharp peaks in the crystallization of the Indium Tin Oxide (ITO) film, due to the micro-electricity and the potential difference, it is easy to cause electrostatic discharge, which burns or even breaks down the ITO traces. The target use time, temperature and atmosphere technology are verified, and the results show that the adjustment of the atmosphere technology is an effective method to solve ESD problem. Through atmosphere technology adjustment, ESD testing performance of LCD reaches the requirements, and the proportion of defective ESD decreases from 18.9% to less than 0.002%. Through improvements, product quality is improved, production cost is reduced and good economic benefits are achieved.