A study on the reflected and hyperspectral mixed-pixel of aquatic plants and water was given by using a orthogonal experimental design with three factors and two levels. The results of F test suggest that for the single factors, the band and the area ratio of mixed-pixel on the reflected and hyperspectral mixed-pixel of the reflection effects are particularly significant, however, the detector angle had no significant effect under these experimental conditions; For the interaction, the band and the area ratio of mixed-pixel, the detector and the area ratio of mixed-pixel, the effects of these two interactions on the reflected and hyperspectral mixed-pixel are also particularly significant, This study did quantitative analysis of the factors affecting the reflected and hyperspectral mixed-pixel character and their interaction, and provided a new method for the indepth study of mixed-pixel.