Spectroscopy and Spectral Analysis, Volume. 36, Issue 3, 896(2016)
Spectral Analysis of Interaction between Human Telomeric G-Quadruplex and Liliflorin A, the First Lignan Derivative Interacted with G-Quadruplex DNA
Human telomeric G-quadruplex is a four-stranded structure folded by guanines (G) via Hoogsteen hydrogen bonding. The ligands which stabilize the G-quadruplex are often telomerase inhibitors and may become antitumor agents. Here, the interaction between a lignan derivative liliflorin A and human telomeric sequence dGGG(TTAGGG)3G-quadruplex HTG21 were examined by CD, FRET, and NMR spectroscopic methods. In addition, Molecular Docking was used to study the binding of liliflorin A to dTAGGG(TTAGGG)3G-quadruplex HTG23. The CD data showed that liliflorin A enhanced HTG21 Tm. The Tm value of G-quadruplex was enhanced 3.2 ℃ by 4.0 μmol·L-1 liliflorin A in FRET. The NMR spectra of HTG21 showed vivid alteration after reacting with liliflorin A in 3 hours. Molecular Docking suggested liliflorin A bound to the wide groove of HTG23 at G9, G10, G16 and G17. Liliflorin A was the first lignan derivative that could stabilize HTG21 selectively and provided a new candidate for antitumor drug design targeting on human telomeric G-quadruplex.
Get Citation
Copy Citation Text
LIU Ting-ting, ZHOU Shuang, JIA Qian-lan, WANG Wen-shu, YAN Xiao-qian, ZHANG Wen-hao, WANG Shuai-qi, JIAO Yu-guo. Spectral Analysis of Interaction between Human Telomeric G-Quadruplex and Liliflorin A, the First Lignan Derivative Interacted with G-Quadruplex DNA[J]. Spectroscopy and Spectral Analysis, 2016, 36(3): 896
Received: May. 8, 2015
Accepted: --
Published Online: Dec. 9, 2016
The Author Email: Ting-ting LIU (liutingting1204@163.com)