Spectroscopy and Spectral Analysis, Volume. 34, Issue 8, 2284(2014)
Quenched Fluorescein: A Reference Dye for Instrument Response Function of TCSPC
Measuring the instrument response function (IRF) and fitting by reconvolution algorithms are routines to improve time resolution in fluorescence lifetime measurements. Iodide ions were successfully used to quench the fluorescence of fluorescein in this study. By systematically adding saturated NaI water solution in basic fluorescein solution, the lifetimes of fluorescein were reduced from 4 ns to 24 ps. The quenched lifetime of fluorescein obtained from the analysis of Time-Correlated Single Photon Counting (TCSPC) measurement agrees well with that from femtosecond frequency up-conversion measurement. In time resolved excitation spectra measurements, the IRF should be measured at various detection wavelengths providing scattring materials are used. This study could not only reduce the complexity of IRF measurement, but also avoid the existing color effect in system. This study should have wide applications in time resolved fluorescence spectroscopy and fluorescence lifetime imaging.
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PAN Hai-feng, DING Jing-xin, LIANG Rong-rong, TAO Zhan-dong, LIU Meng-wei, ZHANG San-jun, XU Jian-hua. Quenched Fluorescein: A Reference Dye for Instrument Response Function of TCSPC[J]. Spectroscopy and Spectral Analysis, 2014, 34(8): 2284
Received: Jul. 18, 2013
Accepted: --
Published Online: Aug. 18, 2014
The Author Email: Hai-feng PAN (hfpan@phy.ecnu.edu.cn)