Noninvasive technology for measuring instantaneously two-dimensional (2D) temperature distributions of flame using two-color planar laser induced fluorescence (PLIF) of OH is investigated. A calibration method is researched and developed. This method is based on the calibration experiments with a laminar premixed flame and thermocouple, and avoids complex calculation and uncertainty of the spectrum parameters. Measurements for a flat burner at ambient temperature under atmospheric pressure are also presented; calibration results are used to diagnose a supersonic combustion in scramjet combustor. The conclusion indicates that this method is useful, and a better precision of calibration can be acquired by correcting the line shapes of the spectral lines and lasers.