Acta Optica Sinica, Volume. 32, Issue 2, 217001(2012)
High Resolution Melting Analyzer Based on High Throughout Fast Detection of Weak Fluorescence
High resolution melting curves analysis (HRM) is a recently developed powerful technique for the detection of gene mutations and SNP analysis. Monitoring the changes in fluorescence from saturated dye labeled on dsDNA, as a PCR products (amplicons) are melted, the minor difference of nucleic acid sequence can be discriminated. Here fast fluorescence detection system based on fiber-optics switch array is designed for fulfilling the requirements of speed and sensitivity. High-density fluorescence data are processed by a series of procedure, such as smoothed, automatic baseline detection, normalizing and differential to temperature, different gene types could be recognized quickly and correctly by the high resolution melting curves.
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Peng Niancai, Zhang Zhenxi, Li Zheng, Zhao Yulong, Jiang Zhuangde. High Resolution Melting Analyzer Based on High Throughout Fast Detection of Weak Fluorescence[J]. Acta Optica Sinica, 2012, 32(2): 217001
Category: Medical optics and biotechnology
Received: Jul. 15, 2011
Accepted: --
Published Online: Jan. 11, 2012
The Author Email: Niancai Peng (pnc@mail.xjtu.edu.cn)