An oxygen optical sensing probe was fabricated by modifying the sensitive film doped with Ru compounds in a holy optical fiber. The structure of this probe was based on the simple holy optical fiber with internal microchannel structure throughout the whole fiber, in which the microchannel structure could be used as a carrier of sensitive materials and a micro-sensing pool and the sensing process was finished in it. The sensing film was deposited in the optical fiber by inhaling the cellulose solution containing ［Ru(dpp)3］2+ directly. Furthermore, the sensing process was based on fluorescence quenching process. As the oxygen could strongly quench the fluorescence of ［Ru(dpp)3］2+ in the sensitive film, the sensing probe could exhibit different fluorescence intensities for different oxygen concentrations. The experimental results show that the designed probe responses to the oxygen in linearity for a full concentration range of 0～100% , and the ratio of I0/I100 (where I0 and I100 respectively represent the fluorescence intensities of the optrode exposed to 100% N2 and 100% O2) is 9.4. Simultaneously, the probe can make a quick response within 50 ms. The experiment also show that the error between experimental value and real value is less than 3% and the resolution can be 2% in a normal pressure.