The influence of electric field penetration on mass spectrometer can be improved by adjusting the reverse bias voltage to improve the resolution of mass spectrometer. To meet the different output waveform requirements of mass spectrometers, this paper proposes a pulse generator that can simultaneously output two pulses of the same voltage amplitude and with opposite polarities, and a negative DC bias voltage is coupled to the high-voltage positive pulse. Only one charging power source is needed to generate both positive and negative pulsed electric fields. This paper analyzes the synchronous driving effect of the series switch, and then solves the problem of uneven charging voltage of the series capacitors by adding the compensation windings and parallel resistors. The difference of charging voltage between four capacitors is within 0.1%. A 4-stage prototype was constructed. This pulse generator can generate a high-voltage positive pulse with amplitude from 0 to 1.5 kV and adjustable pulse width from 2 to 10 µs and coupled with a negative bias voltage with amplitude from 0 to -200 V, and a high-voltage negative pulse with amplitude from 0 to -1.5 kV and adjustable pulse width from 2 to 10 µs. All the rising edges are shorter than 30 ns. The pulse generator can achieve adjustable output voltage, pulse widths and frequencies.