As an emerging channel resource for modern optics, big data, internet traffic and quantum technologies, twisted photons carrying orbital angular momentum (OAM) have been extended their applicable boundary in different media, such as optical fiber and atmosphere. Due to the extreme condition of loss and pressure, underwater transmission of twisted photons has not been well investigated yet. Especially, single-photon tests were all limited at a level of a few meters, and it is in practice unclear what will happen for longer transmission distances. Here we experimentally demonstrate the transmission of single-photon twisted light over an underwater channel up to 55 m, which reach a distance allowing potential real applications. For different order OAM states and their superposition, a good preservation of modal structure and topological charge are observed. Our results for the first time reveal the real transmission performance of twisted photons in a long-distance regime, representing a step further towards OAM-based underwater quantum communication.